Coding

Part:BBa_K3826001:Design

Designed by: Moritz Göbel   Group: iGEM21_Duesseldorf   (2021-10-18)


AT2G43820 coding for the UDP-glycosyltransferase 74F2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 97


Design Notes

This is the naturally occuring sequence. The only consideration was to use cDNA as template to receive the coding sequence excluding introns.


Source

Extracted from Arabidopsis thaliana Col-0 cDNA.

References